Eleven novel microsatellite markers for the Chinese alligator (Alligator sinensis) |
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Authors: | Wei Jing Xiao-Liang Wang Hong Lan Sheng-Guo Fang |
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Institution: | (1) College of Life Sciences, State Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou, 310058, P.R. China;(2) Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals, Ministry of Education, Zhejiang University, Hangzhou, 310058, P.R. China |
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Abstract: | Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, we developed 11 novel microsatellite loci for this rare
species and applied them to examine genetic variation of indigenous alligators from Changxing Nature Reserve and America-born
Chinese alligators. The 11 polymorphic microsatellites presented a total of 31 alleles among 57 individuals scored, yielding
an average of 2.82 alleles per locus. One allele was unique to the American population but four private alleles were detected
in the Changxing population. The average expected and observed heterozygosities were 0.400 and 0.482 for the Changxing alligators
and 0.520 and 0.621 for the America-born individuals, respectively. These microsatellite markers would be useful tools in
the genetic examination of this endangered species. |
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Keywords: | Chinese alligator Microsatellite Genetic diversity Heterozygosity Allele |
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