Differential gene expression of 36-kDa microfibril-associated glycoprotein (MAGP-36/MFAP4) in rat organs |
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Authors: | Tetsuhiko Toyoshima Tetsuya Ishida Nozomu Nishi Ryoji Kobayashi Takehiro Nakamura Toshifumi Itano |
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Institution: | (1) Department of Structure and Functional Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kagawa 761-0793, Japan;(2) Department of Histology and Cell Biology, Faculty of Medicine, Kagawa University, Ikenobe, Miki-cho, Kagawa 761-0793, Japan;(3) Department of Endocrinology, Faculty of Medicine, Kagawa University, Ikenobe, Miki-cho, Kagawa 761-0793, Japan;(4) Department of Signal Transduction Sciences, Faculty of Medicine, Kagawa University, Ikenobe, Miki-cho, Kagawa 761-0793, Japan |
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Abstract: | By using quantitative Western blot analysis and the real time polymerase chain reaction technique, we investigated the differential
gene expression of microfibril-associated glycoprotein (MAGP-36) in rat organs. The gene was expressed highly in sites rich
in elastic fibers, such as aorta, skin, and esophagus. However, MAGP-36 was also expressed highly in some other sites containing
no elastic fibers. In lung and trachea, the expression levels of MAGP-36 mRNA were about seven times higher than those in
other elastic tissues, although the protein abundances were almost at the same levels as other elastic tissues. MAGP-36 seemed
to be secreted outside these organs. In brain, kidney, and spleen, although the expression levels of MAGP-36 mRNA were low,
substantial amounts of MAGP-36 protein were detected. An immunohistochemical study revealed that MAGP-36 was present at the
brush border of the S3 segment of proximal tubules in kidney. Since MAGP-36 is known to bind to mannan, MAGP-36 might be involved
in mannose transport in the S3 segment. Thus, MAGP-36 might be multifunctional and present in a wide variety of sites in various
organs. |
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Keywords: | MAGP-36 MFAP4 AAAP-40 Smith-Magenis syndrome S3 Mannose Rat (Sprague Dawley) |
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