6个中外猪品种的RAPD分析

应用RAPD技术分析了梅山猪、淮南猪、通城猪、八眉猪、合作猪、大白猪的遗传变异。用276个随机引物对6个猪种混合DNA扩增筛选，24个引物产生多态性。此24个引物经重新优化反应条件体系后，对276个个体DNA进行扩增。结果6个猪种的遗传多样性指数分别为0.178672、0.17781、0.15995、0.14549、0.16949和0.14157,群体内平均遗传多样性指数为0.16216，总群体遗传多样性指数为0.2534。基于Rogers公式计算的遗传距离采用NJ和UPGMA法进行聚类分析，结果6个猪种的亲缘关系与它们的地理分布基本一致。 Abstract:The genetic variation of Meishan,Huainan,Tongcheng,Bamei,Hezuo and Largewhite pigs were analyzed by RAPD markers.Twenty-four single polymorphic primers were selected out of 276 primers by amplifying six pool DNA.The index of Shannon were 0.178672,0.17781,0.15995,0.14549,0.16949,0.14159 respectively;Hpop was 0.16216,Hsp was 0.2534.The phylogenetic tree was constructed using NJ and UPGMA.The results indicated that the phenylogenetic relationship of the six pig breeds was consistent with their distribution.

Genetic Variation of Six Breeds of Pigs by RAPD Analysis

The genetic variation of Meishan, Huainan, Tongcheng, Bamei, Hezuo and Largewhite pigs were analyzed by RAPD markers. Twenty-four single polymorphic primers were selected out of 276 primers by amplifying six pool DNA. The index of Shannon were 0.178672, 0.17781,0.15995, 0.14549,0.16949, 0.14159 respectively; Hpop was 0.16216, Hsp was 0.2534. The phylogenetic tree was constructed using NJ and UPGMA. The results indicated that the phenylogenetic relationship of the six pig breeds was consistent with their distribution.