Identification and validation of QTLs for green plant percentage in barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) anther culture |
| |
Authors: | M Muñoz-Amatriaín A M Castillo X W Chen L Cistué M P Vallés |
| |
Institution: | (1) Departamento de Genética y Producción Vegetal, Estación Experimental Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 202, 50080 Zaragoza, Spain;(2) Department of Biochemistry and Molecular Biology, Nankai University, Tianjin, 300071, People’s Republic of China |
| |
Abstract: | In cereals, albinism is a major obstacle to produce doubled haploids (DH) for breeding programs. In order to identify QTLs
for green plant percentage in barley anther culture, a specific population was developed. This population, consisting of 100
DH lines, was generated by crossing the model cultivar for anther culture “Igri” with an albino-producing DH line (DH46) selected
from Igri × Dobla, in search of a maximum segregation for the trait and minimum for the other anther culture variables. A
combination of bulked segregant analysis and AFLP methodology was used to identify markers linked to the trait. A linkage
map was constructed using these AFLPs, together with RAPD, STS and SSR markers. This study identified a new QTL for green
plant percentage on chromosome 3H and confirmed the previously reported one on chromosome 5H. Up to 65.2% of the phenotypic
variance for this trait was explained by the additive effects of these two QTLs. Thirty elite cultivars of barley from different
origin, row type, growth habit and end use, were selected to validate these QTLs. Since two of the markers linked to the QTLs
were AFLPs, we successfully converted them into simple PCR-based SCAR markers. Only the SSR HVM60, on chromosome 3H, was significantly
associated with the trait, explaining near 20% of the phenotypic variance. Among the allelic variants identified for this
marker, HVM60-120bp was associated with the highest values of green plant percentage. |
| |
Keywords: | Albinism Anther culture Barley Doubled haploids Green plant percentage QTLs |
本文献已被 SpringerLink 等数据库收录! |
|