Rapid purification of two thermophilic proteinases using dye-ligand chromatography |
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| Institution: | 1. Thermophile Research Unit, University of Waikato, Hamilton, New Zealand;2. Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK |
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| Abstract: | Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scaleable to 600 l volume. |
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