Genetic diversity analysis in sorghum germplasm as estimated by AFLP, SSR and morpho-agronomical markers |
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Authors: | Nemera Geleta Maryke T Labuschagne Chris D Viljoen |
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Institution: | (1) Department of Plant Sciences, College of Agriculture, Alemaya University, P.O. Box 138, Dire Dawa, Ethiopia;(2) Department of Plant Sciences, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa |
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Abstract: | A comparison of the different methods of the estimation of genetic diversity is important to evaluate their utility as a tool
in germplasm conservation and plant breeding. Amplified fragment length polymorphism (AFLP), microsatellites or SSR and morphological
traits markers were used to evaluate 45 sorghum germplasm for genetic diversity assessment and discrimination power. The mean
polymorphism information content (PIC) values were 0.65 (AFLPs) and 0.46 (SSRs). The average pairwise genetic distance estimates
were 0.57 (morphological traits), 0.62 (AFLPs) and 0.60 (SSRs) markers data sets. The Shannon diversity index was higher for
morphological traits (0.678) than AFLP (0.487) and SSR (0.539). The correlation coefficients obtained by the Mantel matrix
correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated values
of genetic relationship given for AFLP and SSR markers, as well as for morphological and SSR markers were significantly related
(p <0.001). However, morphological and AFLP data showed non-significant correlation (p >0.05). Both data sets from AFLP and SSR allowed all accessions to be uniquely identified; two accessions could not be distinguished
by the morphological data. In summary, AFLP and SSR markers proved to be efficient tools in assessing the genetic variability
among sorghum genotypes. The patterns of variation appeared to be consistent for the three marker systems, and they can be
used for designing breeding programmes, conservation of germplasm and management of sorghum genetic resources. |
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Keywords: | AFLP Genetic diversity Germplasm Morphology Sorghum SSR/microsatellites |
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