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Viruslike particles containing knob-associated histidine-rich protein are secreted into the culture medium of Plasmodium falciparum in vitro cultures
Authors:R Lenstra  A Samso  B Andrieu  J Le Bras  F Galibert
Affiliation:1. Laboratoire d''Hématologie Expérimentale, Hôpital St-Louis, F-75010 Paris, France;2. Departement de Biologie Parasitaire, Institut de Médecine et d''Epidemiologie Tropicales, Hôpital Claude Bernard, F-75019 Paris, France;1. Sydney Institute for Infectious Diseases, School of Medical Sciences, The University of Sydney, Sydney, NSW 2006, Australia;2. Natural History Museum, University of Oslo, 0562 Oslo, Norway;3. Zoological Institute, Department of Environmental Sciences, University of Basel, Vesalgasse 1, 4051 Basel, Switzerland;1. United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA;2. Central Veterinary Research Laboratory, PO Box 597, Dubai, United Arab Emirates;1. Mobile Computing Lab, Department of Computer Science and Engineering, Tripura University, Tripura, India;2. Department of Computer Science, Rama Devi Women’s University, Bhubaneswar, India;3. Department of Computer Science and Engineering, Assam University, Silchar, India;1. Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia;2. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China;1. Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy;2. Research Centre for Viticulture and Enology, CREA, Via XXVIII Aprile 26, 31015 Conegliano, Italy;3. Mycotheca Universitatis Taurinensis (MUT), Department of Life Sciences and Systems Biology, University of Turin, Viale Mattioli 25, 10125 Torino, Italy
Abstract:This report describes the isolation of a viruslike particle from in vitro cultures of the human malaria parasite P. falciparum. Electronmicroscopic observations suggest that the particles are liberated into the culture medium by budding from the erythrocyte membrane. The density of the free particles is 1.16, they contain nucleic acid and two distinct molecular species of the knob-associated Histidine-rich protein. Proteins of the particles are recognized by sera from malaria patients. The previously described knobs may correspond to viral coats inserted in the membrane.
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