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Fluorescence correlation spectroscopy for detecting submicroscopic clusters of fluorescent molecules in membranes |
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| Authors: | Arthur G Palmer III and Nancy L Thompson |
| Institution: | Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, U.S.A. |
| Abstract: | The formation of cell surface receptor clusters has been implicated of confirmed in the mechanism of signal transduction across biological membranes for a variety of processes, including receptor-mediated phagocytosis and endocytosis and cellular response to hormones and neurotransmitters. Flourescence correlation spectroscopy (FCS) is one technique that may provide insight into the kinetics and extent of receptor aggregation. Recent theoretical and experimental developments in FCS for the investigation of submicroscopic clusters of fluorescence molecules are described and the potential applications of the technique to receptor aggregation are reviewed. |
| Keywords: | cell surface receptors cell membranes fluorescence microscopy fluctuations aggregation diffusion |
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