Relative quantitative PCR to assess bacterial community dynamics during biodegradation of diesel and biodiesel fuels under various aeration conditions |
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Authors: | Cyplik Pawe? Schmidt Marcin Szulc Alicja Marecik Roman Lisiecki Piotr Heipieper Hermann J Owsianiak Miko?aj Vainshtein Mikhail Chrzanowski ?ukasz |
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Institution: | a Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, Poznań 60-627, Poland b Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Sk?odowskiej-Curie 2, Poznań 60-965, Poland c Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig 04318, Germany d Pushchino State University, Prospect Nauki 3, Pushchino 142290, Russia e Institute of Biochemistry and Physiology of Microorganisms RAS, Prospect Nauki 5, Pushchino 142290, Russia |
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Abstract: | The degradation of diesel fuel, B20 blend and biodiesel in liquid cultures by a seven-member bacterial consortium was compared under conditions with full aeration or with limited aeration with nitrate added as main electron acceptor. Community dynamics was assessed employing real-time PCR and the ddCt method for relative quantification. Biodegradation rates increased with increasing biodiesel content, but were significantly reduced under conditions with nitrate. Despite large variations in biodegradation rates, magnitude changes in population numbers were typically observed only from zero to one order, regardless the type of fuel and electron acceptor. Only Comamonadaceae and Variovorax sp. distinctly preferred aerobic conditions, and during aerobic growth showed suppression as fuel contained more biodiesel. Thus, the consortium is relatively stable and most of the degraders can shift their metabolism from hydrocarbons to biodiesel. The stability of the consortium is of interest in the context of biodiesel-mediated biodegradation of petroleum hydrocarbons. |
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Keywords: | Anaerobic Biodegradation Biodiesel Consortium ddCt method |
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