Biogenic silver nanoparticles reduce Toxoplasma gondii infection and proliferation in RAW 264.7 macrophages by inducing tumor necrosis factor-alpha and reactive oxygen species production in the cells |
| |
| Affiliation: | 1. Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil;2. Carlos Chagas Institute (ICC/FIOCRUZ/PR), Curitiba, PR, Brazil;3. Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, PR, Brazil;4. Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 Km 380, 86057-970, Londrina, PR, Brazil;5. Laboratory of Pain, Inflammation, Neuropathy, and Cancer, Department of Pathology, Center of Biological Sciences, Londrina State University, Londrina, PR, Brazil;1. Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran;2. Health Sciences Unit, Faculty of Social Sciences, Tampere University, Tampere, Finland;3. HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran;4. Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, USA;5. Department of Epidemiology & Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;6. Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, Tehran, Iran;7. Cancer Biology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran;1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing, China;2. Department of Infectious Medicine, Beijing Children''s Hospital, Capital Medical University, National Center for Children''s Health, Beijing, China;1. Hospital Universitário Júlio Muller, Universidade Federal de Mato Grosso, 78048-902, Cuiabá, Mato Grosso, Brazil;2. Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, 78060-900, Cuiabá, Mato Grosso, Brazil;3. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, 78060-900, Cuiabá, Mato Grosso, Brazil;1. Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha, Qatar;2. Department of Basic Medical Sciences, College of Medicine, QU Health, Qatar University, Doha, Qatar;3. Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha, Qatar;4. Biomedical Research Center, Qatar University, Doha, Qatar;1. Clinical Microbiology Department, Hospital Universitario La Paz, IdiPaz, Paseo de la Castellana, 261, 28046, Madrid, Spain;2. Internal Medicine Service, Infectious Diseases Unit, Hospital Universitario La Paz, Paseo de la Castellana, 261, 28046, Madrid, Spain;1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China;2. Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China;3. Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China |
| |
| Abstract: | Owing to the serious adverse effects caused by pyrimethamine and sulfadiazine, the drugs commonly used to treat toxoplasmosis, there is a need for treatment alternatives for this disease. Nanotechnology has enabled significant advances toward this goal. This study was conducted to evaluate the activity of biogenic silver nanoparticles (AgNp-Bio) in RAW 264.7 murine macrophages infected with the RH strain of Toxoplasma gondii. The macrophages were infected with T. gondii tachyzoites and then treated with various concentrations of AgNp-Bio. The cells were evaluated by microscopy, and culture supernatants were collected for ELISA determination of their cytokine concentration. Treatment with 6 μM AgNp-Bio reduced the infection and parasite load in infected RAW 264.7 macrophages without being toxic to the cells. The treatment also induced the synthesis of reactive oxygen species and tumor necrosis factor-alpha (both pro-inflammatory mediators), which resulted in ultrastructural changes in the tachyzoites and their intramacrophagic destruction. Our findings suggest that AgNp-Bio affect T. gondii tachyzoites by activating microbicidal and pro-inflammatory mechanisms and may be a potential alternative treatment for toxoplasmosis. |
| |
| Keywords: | Toxoplasmosis Treatment Biogenic silver nanoparticles (AgNp-Bio) Cytotoxicity RAW 264.7 cells AgNp-Bio" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" biogenic silver nanoparticles AgNPs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" silver nanoparticles silver nitrate AgCl" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" silver chloride SEM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," _" :" scanning electron microscopy DMEM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0090" }," $$" :[{" #name" :" text" ," _" :" Dulbecco's modified Eagle's medium hydrogen peroxide IL" },{" #name" :" keyword" ," $" :{" id" :" kwrd0110" }," $$" :[{" #name" :" text" ," _" :" interleukin IFN-γ" },{" #name" :" keyword" ," $" :{" id" :" kwrd0120" }," $$" :[{" #name" :" text" ," _" :" interferon-gamma MTT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0130" }," $$" :[{" #name" :" text" ," _" :" 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide PBS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0140" }," $$" :[{" #name" :" text" ," _" :" phosphate-buffered saline qPCR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0150" }," $$" :[{" #name" :" text" ," _" :" real-time quantitative polymerase chain reaction ROS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0160" }," $$" :[{" #name" :" text" ," _" :" reactive oxygen species TEM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0170" }," $$" :[{" #name" :" text" ," _" :" transmission electron microscopy TNF-α" },{" #name" :" keyword" ," $" :{" id" :" kwrd0180" }," $$" :[{" #name" :" text" ," _" :" tumor necrosis factor-alpha |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
