Evaluation of the relative expression of genes associated with adherence after different hours of co-culture between Streptococcus uberis and MAC-T cells |
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| Affiliation: | 1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36, Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;1. Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Buenos Aires, Argentina;2. Servicio Bacteriología, Departamento Laboratorio del Instituto Nacional de Epidemiología “Dr. Juan H. Jara” (INE), Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS), Ministerio de Salud de la Nación, Argentina;3. Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile;1. Department of Blood Transfusion, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China;2. The Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China;3. Department of Intensive Care Unit, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China;1. Hospital Universitário Júlio Muller, Universidade Federal de Mato Grosso, 78048-902, Cuiabá, Mato Grosso, Brazil;2. Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, 78060-900, Cuiabá, Mato Grosso, Brazil;3. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, 78060-900, Cuiabá, Mato Grosso, Brazil;1. Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China;2. Hospital of Stomatology, Jilin University, Changchun 130021, China;3. Shandong Jinzhuji Pharmaceuticals Co. Ltd., Jinan 271100, Shandong, China |
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| Abstract: | Streptococcus uberis is an environmental pathogen associated with subclinical and clinical IMI in both lactating and non-lactating cows. RC19 strain was isolated from a cow with subclinical mastitis, qualitatively classified as moderate biofilm producer in Todd Hewitt medium (THB), and it showed a high value of the adhered bacteria (CFU/ml). Hence, the aims of this study were (a) to determine ability to adhere to and internalize into epithelial cells MAC-T for 1, 2 and 3 h, (b) to evaluate the relative expression of adherence-associated genes from co-cultures of S. uberis with MAC-T cells at 1, 2 and 3 h. We hypothesized that upon contact with bovine mammary epithelial cells, S. uberis upregulates adherence-associated genes encoding adhesins, which enable it a higher adherence to and/or internalization into host cells. Four to six genes increased their R with regard to the control after initial contact with MAC-T cells (group 1) at 1, 2 and 3 h. The highest value of R was observed at 2 h after co-culture between RC19 and MAC-T cells. |
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| Keywords: | Bovine mastitis Adherence Relative expression |
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