Screening of rice cultivars for Cr-stress response by using the parameters of seed germination,morpho-physiological and antioxidant analysis |
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Authors: | Farwa Basit Javaid Akhter Bhat Jiajun Han Yajing Guan Basit Latief Jan Awais Shakoor Saleh Alansi |
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Institution: | 1. Hainan Research Institute, Zhejiang University, Sanya, 572025, China;2. Seed Science Center, Institute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, China;3. International Genome Centre, Jiangsu University, Zhenjiang 212013, China;4. Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia;5. Department of Environment and Soil Sciences, University of Lleida, 25198 Lleida, Spain;6. Botany and Microbiology Department, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia |
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Abstract: | Rice is the most important crop for the majority of population across the world with sensitive behavior toward heavy metals such as chromium (Cr) in polluted regions. Although, there is no information on the Cr resistance phenotyping in rice. Herein, two different groups of rice cultivars (normal, and hybrid) were used, each group with 14 different rice cultivars. Firstly, seed germination analysis was conducted by evaluating various seed germination indices to identify the rice cultivars with greatest seed germination vigor. Furthermore, exposure of chromium (Cr) toxicity to 28 different rice varieties (NV1-NV14, HV1-HV14) caused noticeable plant biomass reduction. Subsequently, NV2, NV6, NV10, NV12, NV13 (normal type), HV1, HV4, HV8, and HV9 (hybrid types) were pragmatic as moderately sensitive varieties, while NV3, NV4, NV9, and NV14 (normal type), HV3, HV6, HV7, and HV13 were observed as moderately tolerant. Although, NV7, and HV10 were ranked most sensitive cultivars, and NV11, and HV14 were considered as most tolerant varieties as compared to the other rice (both groups) genotypes. Afterward, Cr induced reduction in chlorophyll pigments were significantly lesser in HV14 relative to NV11, NV7, and especially HV10, and as a result HV14 modulated the total soluble sugar level as well as reduced ROS accumulation, and MDA contents production by stimulating the antioxidant defense mechanism conspicuously which further reduced the electrolyte leakage as well. Our outcomes provide support to explore the Cr tolerance mechanism in cereal crops as well as knowledge about rice breeding with increased tolerance against Cr stress. |
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Keywords: | Seed germination Photosynthesis Oxidative damage Rice Hydroponic screening Stress tolerance |
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