Comparative study of the antioxidant activity of the essential oils of five plants against the H2O2 induced stress in Saccharomyces cerevisiae |
| |
| Authors: | Khadija Ridaoui Ismail Guenaou Ikram Taouam Mounia Cherki Noureddine Bourhim Abdelaziz Elamrani Mostafa Kabine |
| |
| Affiliation: | 1. Department of Biology, Laboratory of Health and Environment, Faculty of Science Ain Chock, Hassan II University of Casablanca, Morocco;2. Laboratory of Organic Synthesis, Extraction, and Valorization, Faculty of Sciences Aïn Chock, Hassan II University of Casablanca, B.P 2693 Maarif, Casablanca, Morocco |
| |
| Abstract: | The purpose of this work was to investigate the protective effect of five essential oils (EOs); Rosmarinus officinalis, Thymus vulgaris, Origanum compactum Benth., Eucalyptus globulus Labill. and Ocimum basilicum L.; against oxidative stress induced by hydrogen peroxide in Saccharomyces cerevisiae. The chemical composition of the EOs was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The in vitro antioxidant activity was evaluated and the protective effect of EOs was investigated. Yeast cells were pretreated with different concentrations of EOs (6.25–25 µg/ml) for an hour then incubated with H2O2 (2 mM) for an additional hour. Cell viability, antioxidants (Catalase, Superoxide dismutase and Glutathione reductase) and metabolic (Succinate dehydrogenase) enzymes, as well as the level of lipid peroxidation (LPO) and protein carbonyl content (PCO) were evaluated. The chemical composition of EOs has shown the difference qualitatively and quantitatively. Indeed, O. compactum mainly contained Carvacrol, O. basilicum was mainly composed of Linalool, T. vulgaris was rich in thymol, R. officinalis had high α-Pinene amount and for E. globulus, eucalyptol was the major compound. The EOs of basil, oregano and thyme were found to possess the highest amount of total phenolic compounds. Moreover, they have shown the best protective effect on yeast cells against oxidative stress induced by H2O2. In addition, in a dose dependent manner of EOs in yeast medium, treated cells had lower levels of LPO, lower antioxidant and metabolic enzymes activity than cells exposed to H2O2 only. The cell viability was also improved. It seems that the studied EOs are efficient natural antioxidants, which can be exploited to protect against damages and serious diseases related to oxidative stress. |
| |
| Keywords: | Essential oils Yeast Oxidative stress Hydrogen peroxide Antioxidants ANOVA" },{" #name" :" keyword" ," $" :{" id" :" k0040" }," $$" :[{" #name" :" text" ," _" :" Analysis of variance ABTS" },{" #name" :" keyword" ," $" :{" id" :" k0050" }," $$" :[{" #name" :" text" ," _" :" 2,2-azino-bis(3-etilbenzotiazolin)-6-sulfonic acid BHA" },{" #name" :" keyword" ," $" :{" id" :" k0060" }," $$" :[{" #name" :" text" ," _" :" Butylated hydroxyanisole BHT" },{" #name" :" keyword" ," $" :{" id" :" k0070" }," $$" :[{" #name" :" text" ," _" :" Butylated hydroxytoluene BSA" },{" #name" :" keyword" ," $" :{" id" :" k0080" }," $$" :[{" #name" :" text" ," _" :" bovine serum albumin CAT" },{" #name" :" keyword" ," $" :{" id" :" k0090" }," $$" :[{" #name" :" text" ," _" :" catalase DCIP" },{" #name" :" keyword" ," $" :{" id" :" k0100" }," $$" :[{" #name" :" text" ," _" :" 6-Dichlorophenolindophenol DNPH" },{" #name" :" keyword" ," $" :{" id" :" k0110" }," $$" :[{" #name" :" text" ," _" :" 2,4- dinitro-phenylhydrazine reagent DPPH" },{" #name" :" keyword" ," $" :{" id" :" k0120" }," $$" :[{" #name" :" text" ," _" :" 2,2-diphenyl-1-picrylhydrazyl EDTA" },{" #name" :" keyword" ," $" :{" id" :" k0130" }," $$" :[{" #name" :" text" ," _" :" Ethylene diamine tetra acetic acid EOs" },{" #name" :" keyword" ," $" :{" id" :" k0140" }," $$" :[{" #name" :" text" ," _" :" essential oils FID" },{" #name" :" keyword" ," $" :{" id" :" k0150" }," $$" :[{" #name" :" text" ," _" :" flame ionization detector GC" },{" #name" :" keyword" ," $" :{" id" :" k0160" }," $$" :[{" #name" :" text" ," _" :" gas chromatography GC/MS" },{" #name" :" keyword" ," $" :{" id" :" k0170" }," $$" :[{" #name" :" text" ," _" :" gas chromatography-mass spectrometry GR" },{" #name" :" keyword" ," $" :{" id" :" k0180" }," $$" :[{" #name" :" text" ," _" :" Glutathione reductase GSH" },{" #name" :" keyword" ," $" :{" id" :" k0190" }," $$" :[{" #name" :" text" ," _" :" Reduced glutathione GSSG" },{" #name" :" keyword" ," $" :{" id" :" k0200" }," $$" :[{" #name" :" text" ," _" :" Oxidized glutathione Hydrogen peroxide LPO" },{" #name" :" keyword" ," $" :{" id" :" k0220" }," $$" :[{" #name" :" text" ," _" :" Lipid peroxidation MDA" },{" #name" :" keyword" ," $" :{" id" :" k0230" }," $$" :[{" #name" :" text" ," _" :" Malondialdehyde Magnesium chloride NaAc" },{" #name" :" keyword" ," $" :{" id" :" k0250" }," $$" :[{" #name" :" text" ," _" :" Sodium acetate NaCl" },{" #name" :" keyword" ," $" :{" id" :" k0260" }," $$" :[{" #name" :" text" ," _" :" Sodium chloride NADH" },{" #name" :" keyword" ," $" :{" id" :" k0270" }," $$" :[{" #name" :" text" ," _" :" Nicotinamide adenine dinucleotide NADHP" },{" #name" :" keyword" ," $" :{" id" :" k0280" }," $$" :[{" #name" :" text" ," _" :" Nicotinamide adenine dinucleotide phosphate OH" },{" #name" :" keyword" ," $" :{" id" :" k0290" }," $$" :[{" #name" :" text" ," _" :" Hydroxyl radical PBS" },{" #name" :" keyword" ," $" :{" id" :" k0300" }," $$" :[{" #name" :" text" ," _" :" Phosphate buffer saline PCO" },{" #name" :" keyword" ," $" :{" id" :" k0310" }," $$" :[{" #name" :" text" ," _" :" Protein carbonylation PMSF" },{" #name" :" keyword" ," $" :{" id" :" k0320" }," $$" :[{" #name" :" text" ," _" :" Phenylmethylsulfonylfluoride ROS" },{" #name" :" keyword" ," $" :{" id" :" k0330" }," $$" :[{" #name" :" text" ," _" :" Reactive oxygen species RP" },{" #name" :" keyword" ," $" :{" id" :" k0340" }," $$" :[{" #name" :" text" ," _" :" reducing power SD" },{" #name" :" keyword" ," $" :{" id" :" k0360" }," $$" :[{" #name" :" text" ," _" :" standard deviation SDH" },{" #name" :" keyword" ," $" :{" id" :" k0370" }," $$" :[{" #name" :" text" ," _" :" Succinate dehydrogenase SDS" },{" #name" :" keyword" ," $" :{" id" :" k0380" }," $$" :[{" #name" :" text" ," _" :" Sodium dodecyl sulphate SEM" },{" #name" :" keyword" ," $" :{" id" :" k0390" }," $$" :[{" #name" :" text" ," _" :" Standard error of the means SOD" },{" #name" :" keyword" ," $" :{" id" :" k0400" }," $$" :[{" #name" :" text" ," _" :" Superoxide dismutase TBA" },{" #name" :" keyword" ," $" :{" id" :" k0410" }," $$" :[{" #name" :" text" ," _" :" Thiobarbituric acid TBARS" },{" #name" :" keyword" ," $" :{" id" :" k0420" }," $$" :[{" #name" :" text" ," _" :" Thiobarbituric acid reactive substances TCA" },{" #name" :" keyword" ," $" :{" id" :" k0430" }," $$" :[{" #name" :" text" ," _" :" Trichloroacetic acid YPG" },{" #name" :" keyword" ," $" :{" id" :" k0440" }," $$" :[{" #name" :" text" ," _" :" yeast-extract-peptone-glucose |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
