The DNA,RNA and protein composition of the cyanobacterium Anacystis nidulans grown in light- and carbon dioxide-limited chemostats |
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Authors: | Parrott Linda M. Slater J. Howard |
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Affiliation: | (1) Department of Environmental Sciences, University of Warwick, CV4 7AL Coventry, Warwickshire, UK |
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Abstract: | The DNA, RNA and protein content of the cyanobacterium Anacystis nidulans was determined in light-limited and carbon dioxide-limited chemostat cultures over the dilution rate range, D=0.02 h-1 to 0.19 h-1. The macromolecular contents as a percentage of the dry weight and on a per cell basis varied significantly as a function of organism growth rate and the nature of the growth conditions. For both limitations the RNA content per cell increased [20–55 fg RNA (cell)-1] with increasing dilution rate and also showed an increase as a percentage of the dry weight. The DNA content as a percentage of the dry weight showed a 2-fold decrease with increasing dilution rate over the range examined. On a per cell basis DNA reached a peak at D=0.1 h-1 [4.5 fg DNA (cell)-1] for light-limited organisms and at D=0.08 h-1 [8.0 fg DNA (cell)-1] for carbon dioxide-limited organisms. The qRNA increased with increasing dilution rates over the complete growth rate range examined whilst qDNA reached a maximum at D=0.09 to 0.10 h-1. The protein content as a percentage of the dry weight was greater in CO2-limited organisms than light-limited organisms but in both cultures declined as the dilution rate was increased above D=0.10 h-1. |
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Keywords: | Anacystis nidulans Cyanobacterium Chemostat culture RNA DNA Protein |
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