Reaction mechanism of gramicidin S synthetase 1, phenylalanine racemase, of Bacillus brevis |
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Authors: | M Kanda K Hori T Kurotsu S Miura Y Saito |
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Institution: | Department of Biochemistry, Hyogo College of Medicine. |
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Abstract: | We have demonstrated that gramicidin S synthetase 1 (GS 1), phenylalanine racemase EC 5.1.1.11], of Bacillus brevis catalyzes the exchange between a proton in the medium and alpha-hydrogen of phenylalanine in the course of the racemase reaction by using tritiated water or L-phenyl2,3-3H]alanine. GS 1 from some gramicidin S non-producing mutants of B. brevis lacking phenylalanine racemase activity did not catalyze the tritium exchange reaction. The proton exchange between phenylalanine bound as thioester on the GS 1-phenylalanine complex and water in the medium was detected, but 5,5'-dithiobis(2-nitrobenzoic acid)-modified complex lacked both the proton exchange and phenylalanine racemase activity. It is suggested that a base group, probably a sulfhydryl group, on the enzyme functions as proton donor and acceptor during the phenylalanine racemase reaction. |
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