A Simplified Method for Isolating Highly Purified Neurons, Oligodendrocytes, Astrocytes, and Microglia from the Same Human Fetal Brain Tissue |
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Authors: | Malabendu Jana Arundhati Jana Utpal Pal Kalipada Pahan |
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Institution: | (1) Department of Neurological Sciences, Rush University Medical Center, 1735 West Harrison St, Cohn Res Bldg, Suite 320, Chicago, IL 60612, USA;(2) Department of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA;(3) Section of Neuroscience, Department of Oral Biology, University of Nebraska Medical Center College of Dentistry, Lincoln, NE 68583, USA |
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Abstract: | Elucidation of the underlying pathogenic mechanisms leading to apoptosis of neurons and oligodendrocytes and activation of
microglia and astrocytes in different neurodegenerative and neuroinflammatory disorders remains a challenge in neuroscience.
In order to overcome the challenge and find out therapeutic remedies, it is important to study live and death processes in
each and every cell type of the brain. Here we present a protocol of isolating highly purified microglia, astrocytes, oligodendrocytes,
and neurons, all four major cell types of the CNS, from the same human fetal brain tissue. As found in vivo, these primary
neurons and oligodendroglia underwent apoptosis and cell death in response to neurodegenerative challenges. On the other hand,
astroglia, and microglia, cells that do not die in neurodegenerative brains, became activated after inflammatory challenge.
The availability of highly purified human brain cells will increase the possibility of developing therapies for different
neurodegenerative disorders.
M. Jana and A. Jana have equal contribution to the work. |
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Keywords: | Human fetal tissue Neurons Oligodendroglia Microglia Astroglia Neurodegeneration |
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