DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162. |
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Authors: | L S Lin and R J Meyer |
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Institution: | Department of Microbiology, University of Texas, Austin 78712. |
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Abstract: | DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp stem, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted repeat. An analysis of deletions suggests that both start positions must be present for synthesis. A model describing possible early events in replication of plasmid R1162 is presented. |
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