Prolonged apoptosis in mitochondria-rich cells of tilapia (<Emphasis Type="Italic">Oreochromis mossambicus</Emphasis>) exposed to elevated salinity |
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Authors: | Brittany D Kammerer Dietmar Kültz |
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Institution: | (1) Physiological Genomics Group, Department of Animal Science, University of California, Davis, One Shields Avenue, Meyer Hall, Davis, CA 95616, USA |
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Abstract: | The time-course of programmed cell death (apoptosis) during reorganization of gill epithelium in salinity-stressed tilapia
was analyzed using a recently developed method based on laser scanning cytometry (LSC) of dissociated gill cells. Apoptosis
in mitochondria-rich cells (MRC) was distinguished from that in other cell types using Na+/K+ ATPase (NKA) as a cell-specific marker. Caspase 3/7 activity in MRC, assessed using LSC and microplate assays, increased
significantly starting at 6 h of salinity stress and remained elevated for at least 5 days. This time-course of apoptosis
in MRC during acute salinity stress was reflected in elevated apoptotic DNA fragmentation. In parallel to induction of apoptosis,
MRC showed a pronounced shift to G2 phase of the cell cycle, which is indicative of G2/M cell cycle arrest, and an increase
in NKA abundance per MRC. Unlike in MRC, apoptosis was not significantly increased in other gill cell types, although there
was a small transient increase in DNA fragmentation at 6 h. G2 arrest was also observed. Overall, we interpret our data as
evidence for a significant role of apoptosis in the extensive reorganization of MRC populations that takes place during salinity
acclimation, perhaps similar to its well-established role during organismal development. |
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Keywords: | Apoptosis Salinity stress Mitochondria-rich cells Gill epithelial cells Laser scanning cytometry |
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