Toxicity of ethanol during proliferation and adventitious root formation in apple microcuttings in vitro |
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Authors: | G.-J. De Klerk J. Ter Brugge S. Marinova |
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Affiliation: | (1) Centre for Plant Tissue Culture Research, Po Box 85, 2160 Ab Lisse, the Netherlands |
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Abstract: | We have examined the toxicity of ethanol in tissue culture of the apple rootstock ‘Jork 9’. During proliferation through axillary branching, 0.2% (v/v) ethanol slightly stimulated proliferation whereas significant inhibition occurred at concentrations of 0.4 % or higher. In adventitious root formation, significant inhibition occurred at concentrations of 0.1 % or higher. The effect of ethanol was stage-dependent: during the induction period (i.e. from 24 to 72 h after the start of the rooting treatment), there was little or no inhibition. During autoclaving, ethanol evaporated to ca. 50 %. This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Malus domestica micropropagation tissue culture |
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