Purification of bacterial L-methionine gamma-lyase |
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Authors: | T Nakayama N Esaki K Sugie T T Beresov H Tanaka K Soda |
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Affiliation: | Molecular Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 USA |
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Abstract: | A chromatographic procedure using sequential ion-exchange columns is described for separating choline, trimethylamine, trimethylamine oxide, and betaine extracted from marine fish tissues; added exogenous carnitine can also be separated by the system. Choline with its positive charge binds to the AG 50W-X8 (Na+, pH 9) column. The column is first eluted with 0.1 N NaOH to collect trimethylamine, trimethylamine oxide, and betaine; choline is then eluted with 0.5 N NaOH. The amines collected with 0.1 N NaOH are subsequently separated using an AG 50W-X8 (H+, pH 4) column eluted with a linear 0-1 M NaC1 gradient. |
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Keywords: | trimethylamine trimethylamine oxide betaine choline carnitine organic amines |
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