| 伪狂犬病毒gD基因转化马铃薯的初步研究 |
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| 引用本文： | 舒曦,彭昶,王亚琴,胡建广. 伪狂犬病毒gD基因转化马铃薯的初步研究[J]. 西北植物学报, 2009, 29(11) |
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| 作者姓名： | 舒曦 彭昶 王亚琴 胡建广 |
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| 作者单位： | 1. 华南理工大学,生物科学与工程学院,广州,510006
2. 广东省农业科学院作物研究所,广州,510640 |
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| 基金项目： | 广东省科技计划项目，广东省基金项目，广东省国际合作项目 |
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| 摘 要： | 以可直接饲用的马铃薯作为伪狂犬病病毒糖蛋白基因gD的表达植物,探索用马铃薯茎段作为外植体的再生体系和遗传转化体系.结果表明:添加2 mg/L ZT和0.1 mg/L NAA的MS培养基,可使马铃薯茎段愈伤组织诱导率达70%,出芽率达38%;添加0.5 mg/L的硝酸银可明显减少愈伤组织在继代过程中的褐化程度,并且促进其分化.在农杆菌的菌液OD_(600)值为0.1～0.2,侵染茎段1 h,共培养3 d的条件下转化效果最好,抗性愈伤率达到52.3%.实验共获得21株转化植株,挑选7株进行RT-PCR检测,结果证明gD基因已被整合到马铃薯基因组中.
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| 关 键 词： | 马铃薯 伪狂犬病病毒 gD基因 根癌农杆菌介导 遗传转化 |
Primary Study on Transformation of gD Gene from the Pseudorabies Virus into Potato |
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| SHU Xi,PENG Chang,WANG Ya-qin,HU Jian-guang. Primary Study on Transformation of gD Gene from the Pseudorabies Virus into Potato[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(11) |
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| Authors: | SHU Xi PENG Chang WANG Ya-qin HU Jian-guang |
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| Affiliation: | SHU Xi1,PENG Chang1,WANG Ya-qin1*,HU Jian-guang2 (1 School of Bioscience , Bioengineering,South China University of Technology,Guangzhou 510006,China,2 Institute of Crop Sciences,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China) |
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| Abstract: | In the research we used potato as bioreactor to express Glycoprotein D gene(gD) from the pseudorabies virus and studied its regeneration system and genetic transformation system with potato stem as the explant.The results indicated that adding 2 mg/L ZT and 0.1 mg/L NAA to the MS medium can increase the rate of callus induction to 70% and the rate of shoot regeneration to 38%.Adding 0.5 mg/L AgNO3 can prevent callus from browning and promote callus regeneration.The optimal transformation system was establis... |
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| Keywords: | potato pesuedorabies virus gD gene mediated by Agrobacterium tumefacines genetic transformation |
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