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Glucose biosensor based on nano-SiO2 and "unprotected" Pt nanoclusters |
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| Authors: | Yang Haipeng Zhu Yongfa |
| Affiliation: | aDepartment of Chemistry, Tsinghua University, Beijing 100084, PR China bCollege of Materials Science and Engineering, Shenzhen University, Shenzhen Key Laboratory of Special Functional Materials, Shenzhen 518060, PR China |
| Abstract: | The “unprotected” Pt nanoclusters (average size 2 nm) mixed with the nanoscale SiO2 particles (average size 13 nm) were used as a glucose oxidase immobilization carrier to fabricate the amperometric glucose biosensor. The bioactivity of glucose oxidase (GOx) immobilized on the composite was maintained and the as-prepared biosensor demonstrated high sensitivity (3.85 μA mM−1) and good stability in glucose solution. The Pt–SiO2 biosensor showed a detection limit of 1.5 μM with a linear range from 0.27 to 4.08 mM. In addition, the biosensor can be operated under wide pH range (pH 4.9–7.5) without great changes in its sensitivity. Cyclic voltammetry measurements showed a mixed controlled electrode reaction. |
| Keywords: | “Unprotected” Pt nanoclusters Glucose oxidase Glucose biosensor Nanoscale SiO2 |
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