Incorporation and Degradation of C and H-labeled Thymidine by Sugarcane Cells in Suspension Culture |
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Authors: | Lesley S M |
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Institution: | Chemistry and Biology Research Institute, Research Branch, Agriculture Canada, Ottawa K1A 0C6 Ontario. |
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Abstract: | Sugarcane cells growing in suspension culture degrade exogenous thymidine, releasing thymine. Thymine is not utilized for DNA synthesis. Thymine is rapidly catabolized to β-aminoisobutyric acid which is found within the cell. Thymidine in the medium is used for DNA synthesis. The label of 2-14C]thymidine is lost as 14CO2, but the label of 3H]methylthymidine is found in the cell as 3H]β-aminoisobutyric acid, some of which is used for the synthesis of other cell components. The degradation of thymidine can be partially inhibited by addition of certain substituted pyrimidines. |
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