Improved gene disruption method for Torulaspora delbrueckii

PCR-based disruption cassettes are one of the most commonly used strategies for gene targeting in Saccharomyces cerevisiae . The efficiencies of gene disruption using this conventional method are highly variable among species, and often quite low with nonconventional yeasts. Here we describe an improved strategy to obtain deletion mutants in baker's yeast Torulaspora delbrueckii , one of the most abundant non- Saccharomyces species, present in home-made corn and rye bread dough.