Experiments and strategies for the assignment of fully13 C/15N-labelled polypeptides by solid state NMR |
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Authors: | Suzana K. Straus Tobias Bremi Richard R. Ernst |
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Affiliation: | (1) Laboratorium für Physikalische Chemie, ETH Zentrum, CH-8092 Zürich, Switzerland |
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Abstract: | High-resolution heteronuclear NMR correlation experiments and strategies are proposed for the assignment of fully13 C/15N-labelled polypeptides in the solid state. By the combination of intra-residue and inter-residue13 C-15N correlation experiments with13 C-13C spin-diffusion studies, it becomes feasible to partially assign backbone and side-chain resonances in solid proteins. The performance of sequences using 15N instead of13 C detection is evaluated regarding sensitivity and resolution for a labelled dipeptide (L-Val-L-Phe). The techniques are used for a partial assignment of the 15N and 13C resonances in human ubiquitin. |
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Keywords: | assignment fully 13C/15N-labelled biomolecules heteronuclear correlation homonuclear decoupling resolution enhancement solid state NMR |
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