Preparation of girdle lamella-containing chloroplasts from the diatom Phaeodactylum tricornutum |
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| Authors: | Murata Norio; Kume Naho-aki; Okada Yasuko; Hori Terumitsu |
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| Institution: | 1Department of Biology, University of Tokyo Komaba, Meguro-ku, Tokyo 153, Japan
2Institute of Biological Sciences, University of Tsukuba Sakura-mura, Ibaraki 300-31, Japan |
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| Abstract: | Chloroplasts were isolated from the diatom Phaeodactylum tricornutumby French press treatment and centrifugation. Electron micrographsof the isolated chloroplasts indicated that they lacked mostof the envelope membranes but retained the lamellar structurecharacteristic of the diatom chloroplast; three thylakoids weregrouped to form a band which transversed the chloroplast. Agirdle lamella also composed of three thylakoids surroundedthese transversal lamellae. The isolated chloroplasts were activein photosynthetic electron transport reactions including theHill reaction, the Mehler reaction and the system I reaction. (Received May 18, 1979; ) |
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