The extraction of plasma 3-hydroxy-17-oxo steroid sulphates and the measurement of the constituent dehydroepiandrosterone sulphate and androsterone sulphate |
| |
| Authors: | Jean McKenna and A E Rippon |
| |
| Institution: | Medical Research Council Pathology of Steroids Research Unit, Jessop Hospital for Women, Sheffield 3 |
| |
| Abstract: | 1. A simple method for the extraction of 17-oxo steroid sulphates of plasma is described; glucosiduronates and orthophosphates are extracted, but to a smaller extent. 2. Four methods of analyses of the extracts are given and are relatively simple. Three of these are specific for steroid sulphates and two measure the sulphate conjugates directly. 3. Values for dehydroepiandrosterone sulphate and androsterone sulphate concentrations of normal and pathological plasmas are given. |
| |
| Keywords: | |
|
