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Lectin-like components in the macronuclear replication bands of Euplotes eurystomus
Authors:D E Olins  A L Olins  A P Sève  C A Bourgeois  J Hubert  M Monsigny
Institution:University of Tennessee Oak Ridge Graduate School of Biomedical Sciences 37830.
Abstract:Upon incubation with fluoresceinylated neoglycoproteins, isolated macronuclei from the ciliated protozoan Euplotes eurystomus display different labelling patterns depending on the nature of the sugar bound to the neoglycoproteins. Specific sugar-binding components (i.e., lectin-like molecules) are associated with presumed nucleoli and with the macronuclear replication bands. This is the first demonstration that DNA synthesis and sugar-binding components are co-localized in an eukaryotic cell.
Keywords:nuclear lectin  neoglycoprotein  DNA synthesis  nucleolus  fluorescenc microscopy
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