Lectin-like components in the macronuclear replication bands of Euplotes eurystomus |
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Authors: | D E Olins A L Olins A P Sève C A Bourgeois J Hubert M Monsigny |
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Institution: | University of Tennessee Oak Ridge Graduate School of Biomedical Sciences 37830. |
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Abstract: | Upon incubation with fluoresceinylated neoglycoproteins, isolated macronuclei from the ciliated protozoan Euplotes eurystomus display different labelling patterns depending on the nature of the sugar bound to the neoglycoproteins. Specific sugar-binding components (i.e., lectin-like molecules) are associated with presumed nucleoli and with the macronuclear replication bands. This is the first demonstration that DNA synthesis and sugar-binding components are co-localized in an eukaryotic cell. |
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Keywords: | nuclear lectin neoglycoprotein DNA synthesis nucleolus fluorescenc microscopy |
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