Mannanase activity of Endoglucanase III from Trichoderma reesei QM9414 |
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| Authors: | R. Macarró n, C. Acebal, M. P. Castilló n M. Claeyssens |
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| Affiliation: | (1) Centro de Investigación Básica, SmithKline Beecham, Tres Cantos, S.A., Spain;(2) Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Biológicas, Universidad Complutense, Madrid, Spain;(3) Vakgroep Biochemie-Fysiologie-Microbiologie, Faculteit Wetenschappen, Universiteit Gent, K. L. Ledeganckstraat, 35, B-9000 Ghent, Belgium |
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| Abstract: | Summary The cellulase and  -mannanase activities of Endoglucanase III from Trichoderma reesei can be attributed to the same active site. Reversible and irreversible inhibitors affect similarly both activities. Galactomannan is a competitive substrate for a specific endoglucanase substrate. Km values are similar for mannan and carboxymethylcellulose but kcat values are ten times higher for the latter. |
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