Noninvasive NMR studies of metabolism in cultured Catharanthus roseus cells |
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Authors: | Hans J Vogel Peter Lundberg Kirsten Bagh |
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Institution: | 1. Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, Alberta, Canada
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Abstract: | Summary Nuclear magnetic resonance (NMR) spectroscopy provides a unique modality for the study of tissue-cultured plant cells. One
of its major attractions is that it allows noninvasive studies of plant material. In addition, it can provide insight into
the pH in the vacuole and cytoplasm, and into the compartmentalization of certain metabolites. In this review we show how
phosphorus-31 NMR is used to study intracellular pH, phosphate uptake and storage, and energy metabolism in suspension cells
of Catharanthus roseus. In addition, multinuclear NMR studies of the uptake of ammonium and the gradients of K+ over the membrane are discussed as well. The use of two-dimensional NMR for the study of whole cell extracts is also described.
Finally, we show how nitrogen-14 and nitrogen-15 NMR are used to obtain information about the assimilation of inorganic sources
in developing carrot somatic embryos. These NMR studies provide a unique insight into the metabolism of tissue-cultured plant
cells. |
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Keywords: | Catharanthus roseus phosphate uptake ammonium uptake glutamine synthase GS/GOGAT pH control elicitor carrot somatic embryos |
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