Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions |
| |
Authors: | Una Riekstina Ruta Muceniece Inese Cakstina Indrikis Muiznieks Janis Ancans |
| |
Institution: | (1) Faculty of Medicine, University of Latvia, 1a Sarlotes Street, Riga, 1001, Latvia;(2) Faculty of Biology, University of Latvia, 4 Kronvalda blvd., Riga, 1586, Latvia |
| |
Abstract: | This study investigated conditions for optimal in vitro propagation of human skin-derived mesenchymal stem cells (S-MSC).
Forty primary skin-derived precursor cell (SKP) cultures were established from both male and female donors (age 29–65 years)
and eight of them were randomly selected for in-depth characterization. Effects of basic fibroblast growth factor (FGF-2),
epidermal growth factor (EGF), leukemia inhibiting factor (LIF) and dibutyryl-cyclic adenosine monophosphate (db-cAMP) on
S-MSC proliferation were investigated. Primary SKP cultures were >95% homogenous for CD90, CD73, and CD105 marker expression
enabling to classify these cells as S-MSC. FGF-2 dose-dependent stimulation was observed in low serum medium only, whereas
EGF neither stimulated S-MSC proliferation nor potentates the effect of FGF-2. Pronounced donor to donor differences among
S-MSC cultures were observed in 3-day proliferation assay. This study demonstrates that homogenous S-MSC populations can be
reproducibly isolated from individual donors of different age. Optimal cell culture conditions for in vitro propagation of
S-MSC are B27 supplemented or low serum media with FGF-2 (4 ng/ml). EGF and LIF as well as db-cAMP are dispensable for S-MSC
proliferation. |
| |
Keywords: | Skin-derived mesenchymal stem cells (S-MSC) Proliferation FGF-2 EGF db-cAMP FBS FACS |
本文献已被 SpringerLink 等数据库收录! |
|