Spectroscopic characterization of rat kidney Hg,Cu-metallothionein |
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| Authors: | J A Szymańska A J Zelazowski M J Stillman |
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| Affiliation: | Section on Intermediary Metabolism Laboratory of Biochemistry and Metabolism National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health Bethesda, Maryland 20205 U.S.A. |
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| Abstract: | Absorption, circular dichroism (CD), magnetic circular dichroism (MCD) and emission spectra are reported for rat kidney Hg,Cu-metallothionein isoform 3 isolated following induction of the metallothionein with HgCl2. While the absorption spectrum is featureless, both the CD and MCD spectra show resolved bands that arise from the Cu-thiolate and Hg-thiolate groups. The emission spectrum at 77 K is much more complicated than would be expected for a copper (I)-containing metallothionein. It is suggested the emission only arises from the copper-thiolate groups but that the presence of the mercury results in copper ions in several different environments depending on the nature of the nearest neighbour. |
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| Keywords: | address correspondence to this author |
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