Experimental Infection of Mice with Yersinia enterocolitica Serotype O9 by Oral and Parenteral Routes: Spreading and Enterotropism of Virulent Yersiniae |
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Authors: | Alfonso Ruiz-Bravo Encarnacion Moreno Antonio Sampedro Maria Jimenez-Valera |
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Institution: | (1) Departamento de Microbiologia, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain , ES |
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Abstract: | An isogenic pair of Yersinia enterocolitica serotype O9 strains, with and without virulence plasmid, was used to study the plasmid role in the infection of BALB/c mice
by oral, intraperitoneal, and intravenous routes. The plasmid-bearing strain, but not its plasmid-less derivative, caused
enteric infection after challenge by all three routes. The virulence plasmid did not influence the peritoneal clearance of
yersiniae, but only the plasmid-bearing yersiniae were able to move from the peritoneal cavity to the bloodstream, and thus
they spread to spleen and liver. Moreover, plasmid-bearing yersiniae were able to move from the liver to the gallbladder,
and they shed in bile into the intestine. Western blot analysis of antibody responses to chromosomally encoded outer membrane
proteins revealed similar patterns with sera from mice challenged with each one of the two strains by intraperitoneal route.
In contrast, only the plasmid-bearing strain elicited an antibody response to these antigens in mice challenged by oral route.
Although mice experimentally infected with plasmid-bearing O9 yersiniae developed an enteric infection, irrespective of the
inoculation route, differences between the first steps in infection by oral and parenteral routes may be important, especially
when the infection model is used as an approach to study the yersinia-host interactions.
Received: 31 August 1998 / Accepted: 13 November 1998 |
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