Rapid Elevation of Calcium Concentration in Cultured Dorsal Spinal Cord Astrocytes by Corticosterone |
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Authors: | Junwei Zeng Min Li Zhi Xiao Yuanshou Chen Quanzhong Chang Hong Tian Huan Jin Xiaohong Liu |
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Institution: | 1. Department of physiology, Zunyi Medical College, 201 Dalian Street, Zunyi, Guizhou, 563000, People’s Republic of China
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Abstract: | In addition to the classic genomic effects, increasing evidence suggests that GC can generate multiple rapid effects on many tissues and cells through nongenomic pathway. In the present study, the effects of corticosterone (CORT) on the intracellular calcium concentration (Ca2+]i) in cultured dorsal spinal cord astrocytes were detected with confocal laser scanning microscopy using fluo-4/AM as a calcium fluorescent indicator that could monitor real-time alterations of Ca2+]i. CORT (0.01–10 μM) caused a rapid increase in Ca2+]i with a dose-dependent manner in cultured dorsal spinal cord astrocytes. The action of CORT on astrocytic Ca2+]i was blocked by pertussis toxin (a blocker of G protein activation, 100 ng/ml), but was unaffected by RU38486 (glucocorticoid receptor antagonist, 10 μM). In addition, cycloheximide (protein-synthesis inhibitor, 10 μg/ml) pretreatment could not impair the CORT-evoked Ca2+]i elevation. Furthermore, Ca2+ mobilization induced by CORT was abolished by chelerythrine chloride (protein kinase C inhibitor, 10 μM), but was not impaired by H89 (protein kinase A inhibitor, 10 μM). These observations suggest that a nongenomic pathways might be involved in the effect of CORT on Ca2+]i in cultured dorsal spinal cord astrocytes. In addition, our results also raise a possibility that a putative pertussis toxin-sensitive mGCR (G-protein-coupled membrane-bound glucocorticoid receptor) and the downstream activation of protein kinase C may be responsible for CORT-induced Ca2+ mobilization in cultured dorsal spinal cord astrocytes. |
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