Resistance of transformed and non-transformed oilseed rape cv. HM-81 to the infection with cauliflower mosaic,turnip yellow mosaic and turnip mosaic viruses |
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Authors: | J ?pak Jana Dusbábková Darina Kubelková J Ne?ásek |
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Institution: | 1. Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Brani?ovská 31, 370 05, ?eské Budějovice, Czechoslovakia
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Abstract: | Resistance of transformed and non-transformed spring oilseed rape cv. HM-81 to the infection with cauliflower mosaic virus
(CaMV), turnip yellow mosaic virus (TYMV) and turnip mosaic virus (TuMV) was studied, to determine the influence of transformation
on susceptibility of plants to viruses. For experiments the non-segregating R 1 generation of primary transformant HM-81-JZ
and control plants of cv. HM-81 were used. The primary transformant was obtained by inoculation of stems withAgrobacterium rhizogenes 15834. All transformed plants of R 1 generation had typically „transformed“ phenotype. No significant differences were revealed
in the resistance of both transformed and non-transformed plants to each virus, as proved by qualitative and quantitative
ELISA and visual evaluation of symptoms. Transformed plants infected with turnip yellow mosaic virus showed significantly
lower reduction of green mass yield than non-transformed. In the case of CaMV and TuMV infection reduction of yield of transformed
and non-transformed plants was almost the same. |
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