Isolation and Characterization of a Pseudomonas Oleovorans Degrading the Chloroacetamide Herbicide Acetochlor |
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Authors: | Jun Xu Xinghui Qiu Jiayin Dai Hong Cao Min Yang Jing Zhang Muqi Xu |
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Institution: | (1) Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxi Lu, Haidian, P.O. Box 94, 100080 Beijing, P.R. China;(2) Research Centre of Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, P.R. China;(3) Graduate School of the Chinese Academy of Sciences, Beijing, P.R. China |
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Abstract: | To date, no pure bacterial cultures that could degrade acetochlor have been described. In this study, one strain of microorganism
capable of degrading acetochlor, designated as LCa2, was isolated from acetochlor-contaminated soil. The strain LCa2 is Pseudomonas oleovorans according to the criteria of Bergey’s manual of determinative bacteriology and sequence analysis of the partial 16S rRNA
gene. Optimum growth temperature and pH were 35 °C and 8.0, respectively. The strain could degrade 98.03% of acetochlor treated
at a concentration of 7.6 mg l−1 after 7 days of incubation and could tolerate 200 mg l−1 of acetochlor. When the acetochlor concentration became higher, the degradation cycle became longer. The acetochlor biodegradation
products were identified by GC–MS based on mass spectral data and fragmentation patterns. The main plausible degradative pathways
involved dechlorination, hydroxylation, N-dealkylation, C-dealkylation and dehydrogenation. |
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Keywords: | acetochlor degradation isolation Pseudomonas oleovorans |
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