Effects of amino acids, nitrate, and ammonium on the growth and taxol production in cell cultures of Taxus yunnanensis |
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Authors: | Yong-Qin Chen Fei Yi Min Cai Jian-Xin Luo |
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Institution: | (1) Present address: Faculty of Life Sciences, Hubei University, Wuhan, Hubei Province 430062, China, University of Connecticut, 105 Ahern Lane, Storrs, CT 06269, USA(e-mail;(2) China |
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Abstract: | The effects of concentration of amino acids, nitrate, and ammonium on the growth and taxol production in cultures of cell line TY-21 of Taxus yunnanensis were investigated. Addition of 20 different amino acids each at 15–20 mg l–1 to B5 medium significantly improved callus growth but inhibited taxol formation in the cultures. The optimum nitrate concentration was 20–30 mM for both growth and taxol production. Ammonium greatly suppressed growth but strongly promoted taxol formation in the cells when it was the sole inorganic nitrogen in the medium. Culturing the suspension cells in nitrate-containing medium for 15 days and then in a medium in which ammonium was the sole inorganic nitrogen for 7 days increased taxol yield by 104%, reaching up to 28.1 mg l–1. |
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Keywords: | Amino acids Ammonium Nitrate Taxol Taxus yunnanensis |
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