Preparation of monoclonal antibodies against planarian organs and the effect of fixatives |
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| Authors: | Takao Shinozawa Hajime Kawarada Kazuhisa Takezaki Hideaki Tanaka Kinji Inoue |
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| Institution: | (1) Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, 376 Gunma, Japan;(2) Department of Pharmacology, Faculty of Medicine, Gunma University, Maebashi, 371 Gunma;(3) Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371 Gunma |
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| Abstract: | Monoclonal antibodies were obtained against all part of the body of the planarian Dugesia japonica japonica except tthe nerve cord, though parts of the enteric canal were also non reactive. The effect of a variety of fixatives (acetone, Bouin, formalin) used on planarian tissues prior to screening with antibodies, was assessed. |
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| Keywords: | planarian monoclonal antibody fixing reagent |
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