Immunoanalysis of calf uterine progesterone receptor: Modulation of receptor-associated 90 kDa heat-shock protein |
| |
Authors: | Cliff Hurd Makoto Nakao Naomi Eliezer V K Moudgil |
| |
Institution: | (1) Fundaçao Hemocentro de Sao Paulo-FIHSP A v. Dr. Eneas de Carvalho Aguiar, 255, PAMB, B1.12 e 6, CEP: 05403, Sao Paulo-SP, Brazil;(2) Present address: New York University Medical Center, 550-560, 1st Ave., 10016 New York, N. Y.;(3) Departamento de Bioquimica, Instituto de Quimica da Universidade de Sao Paulo, Cx. Postal 20780, Sao Paulo-SP, Brazil;(4) Faculdade de Ciências Farmacêuticas da Universidade de Sao Paulo, Cx. Postal 30786, Sao Paulo-SP, Brazil;(5) Departamento de Análises Clínícas e Toxicologicas, Faculdade Cx. Postal 30786, CEP: 05508 Sao Paulo-SP, Brazil |
| |
Abstract: | Porphyrias are inherited and acquired diseases of erythroid or hepatic origin, in which there are defects in specific enzymes of the heme biosynthetic pathway. In patients with intermittent acute porphyria and lead poisoning the erythrocytic activities of superoxide dismutase and glutathione peroxidase are reported to be increased. Our studies demonstrated that d-aminolevulinic acid, a heme precursor accumulated in both diseases, undergoes enolization at pH < 7.0 before it autoxidizes. The autoxidation of d-aminolevulinic acid, in the presence or absence of oxyhemoglobin has been proposed as a source of oxy and carbon-centred radicals in the cells of intermittent acute porphyria and saturnism carriers. Thus, the increased levels of antioxidant enzymes can be viewed as an intracellular response against the deleterious effects of these extremely reactive species. |
| |
Keywords: | porphyrias intermittent acute porphyria lead poisoning d-aminolevulinic acid oxygen free radicals |
本文献已被 SpringerLink 等数据库收录! |
|