Purification and characterization of a lectin from Phaseolus acutifolius var. latifolius |
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| Authors: | F Vargas-Albores G de la Fuente C Agundis F Córdoba |
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| Affiliation: | Unidad de Medicina Experimental, Facultad de Medicina, C.U., Universidad Nacional Autónoma de México, Cuernavaca, Morelos. |
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| Abstract: | The lectin from Phaseolus acutifolius var. latifolius has been purified to homogeneity by affinity chromatography using stroma-sephadex, gel filtration and electrofocusing. The purified lectin consists of four subunits of 21 Kd molecular mass each one with 7.4% w/w total carbohydrate. Red cells of human and animal species, are agglutinated but are not inhibited by a series of sugars. Mitogenicity and immunosuppressive activities were demonstrated. Agglutination requires magnesium and calcium ions. |
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