Genotyping of Bison bonasus K-casein gene following DNA sequence amplification |
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| Authors: | B Burzynska J Topczewski |
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| Affiliation: | Department of Genetics and Animal Breeding, Warsaw Agricultural University, 05–840 Brwinow, Przejazd 4, Poland;Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02–106 Warsaw, Pawinskiego 5a, Poland |
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| Abstract: | DNA isolated from 30 Bison bonasus blood samples was used for amplification of a kappacasein gene fragment to detect possible polymorphism. Bovine K-caseins exist in A, B and E variants. In the European bison from Poland, we have found only BB genotypes. Sequencing of the polymerase chain reaction product revealed further polymorphisms typical for B. bonasus. |
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| Keywords: | allelic variation Bison bonasus kappa-casein PCR |
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