Biosynthesis of prostaglandins in human ovarian tissues

Experiments in vitro demonstrate, that there are different patterns of PG-biosynthesis in the corpus luteum and in the follicles containing cortical substance of the human ovary. In the follicles 6-keto-F₁α. the transformation product of prostacyclin, is the main fraction; prostaglandins F₂α and E 2 being of inferior importance with regard to their amounts. The formation of the corpus luteum is in close correlation with a strongly increased prostaglandin E₂ and a diminished prostacyclin biosynthesis; prostaglandin F₂α hardly seems to be involved in this process.By means of indomethacin the formation of all three examined prostaglandins can be prevented almost completely, in the cortical substance as well as in the corpus luteum. LH (or HCG) at concentrations ranging from 2 ng to 20 μg per ml homogenate produce no stimulating effect of statistical significance on the rate of biosynthesis in both tissues.