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Immunohistochemical localization of minor gangliosides in the rat central nervous system |
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| Authors: | Kotani, Masaharu Kawashima, Ikuo Ozawa, Hideki Ogura, Kiyoshi Ishizuka, Ineo Terashima, Toshio Tai, Tadashi |
| Affiliation: | Department of Tumor Immunology, The Tokyo Metropolitan Instltute of Medical Science, Honkomagome Bunkyo-ku, Tokyo 113, Japan 1Department of Biochemistry, Teikyo University School of Medicine Kaga, Itabashi-ku. Tokyo 173, Japan 2Department of Anatomy, The Tokyo Metropolitan Institute for Neuroscience, Musashidai Fuchu, Tokyo 183, Japan |
| Abstract: | We previously described the differential distribution of majorgangliosides (GM1, GD1a, GD1b, GT1b and GQ1b) in adult rat braindetected by specific antibodies (Kotani,M., Kawashima,I., Ozawa,I.,Terashima,T. and Tai,T. Glycobiology, 3, 137146, 1993).We report here the distribution of minor gangliosides in theadult rat brain by an immunofluorescence technique with mousemonoclonal antibodies (MAbs). Ten MAbs (GMR6, GMB28, GMR11,GMR19, GMR2, GMR7, GGR51, AMR10, NGR54 and NGR53) that specificallyrecognize GM3, GM2, GT1a, GD3, O-Acdisialoganglioside, GD2,GM1b, GM4, IV3NeuAc |
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