Inositol 1,4,5-trisphosphate as fertilization signal in plants: testcase Chlamydomonas eugametos |
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Authors: | A. Musgrave F. Schuring T. Munnik K. Visser |
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Affiliation: | (1) Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands |
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Abstract: | Within the first 2 h of sexual reproduction, gametes of the green alga Chlamydomonas eugametos agglutinate, fuse via their mating structures, de-agglutinate and swim off as vis-à-vis pairs. During this period, increases in intracellular inositol 1,4,5-trisphosphate levels and changes in polyphosphoinositide synthesis were associated with cell fusion. The protein-kinase-C inhibitor staurosporine (0.1–0.2 M) inhibited the de-agglutination of pairs and therefore prevented them swimming away, while earlier stages of mating such as agglutination or cell fusion were unaffected. The results suggest that inositol 1,4,5-trisphosphate and diacylglycerol are fertilization signals in C. eugametos. The idea that they could also be fertilization signals in higher plants is discussed in relation to in vitro embryogenesis.Abbreviations DAG diacylglycerol - InsP3 inositol 1,4,5-trisphosphate - mt+/mt– mating-type plus or minus - PKC protein kinase C - PtdOH phosphatidic acid - PtdInsP phosphatidylinositol - 4-phosphate PtdInsP2 phosphatidylinositol 4,5-bisphosphate |
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Keywords: | Chlamydomonas Fertilization Gamete fusion Inositol 1,4,5-trisphospate Plant cell-signalling Polyphosphoinositides |
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