Colicinogenic mutant of ColE1 plasmid that fails to confer immunity to colicin E1. |
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Authors: | S Maeda K Shimada Y Takagi |
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Affiliation: | Department of Biochemistry, Kyushu University 60, School of Medicine, Fukuoka 812 Japan |
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Abstract: | Insertion of the ampicillin transposon (Tn3) into ColE1 DNAs causes various mutations in the plasmids. Escherichia coli K-12 cells carrying one of these mutants showed novel properties; they were sensitive to colicin E1 and were able to produce active colicin E1. The site and the orientation of Tn3 insertion in this mutant ColE1 DNA were determined by heteroduplex analysis and by enzymatic digestion with restriction endonucleases. The potential usefulness of this mutant ColE1 DNA as a cloning vehicle is discussed. |
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Keywords: | λ phage specialized transduction cloning vehicle |
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