肌球蛋白单克隆抗体标记大鼠心肌坏死的研究 |
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引用本文: | 朱筱娟,茂昆,赵建增,李荣文,凤桐,侯立中,杨同书. 肌球蛋白单克隆抗体标记大鼠心肌坏死的研究[J]. 动物学报, 2001, 47(3): 351-354 |
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作者姓名: | 朱筱娟 茂昆 赵建增 李荣文 凤桐 侯立中 杨同书 |
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作者单位: | 1. 吉林大学生物工程研究所;东北师范大学遗传与细胞研究所 2. 吉林大学第三临床医学院, 3. 吉林大学生物工程研究所, |
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基金项目: | 国家自然科学基金,39370655, |
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摘 要: | Monoclonal antibody (MAb) attained from hybridoma 1A5 was against human cardiac myosin heavy chains (CMHC) and didn't react with smooth muscle. It was labeled with Na 125 I and injected to the rats suffered from acute myocardial necrosis, which were treated by isoproylarterenol. After injection, the rats were studied from the 4 th to the 48 th hours by relative radioactivity in blood, thyroid gland, stomach, liver, lung, muscle and heart, respectively. The results indicated that 125 I CMHC MAb could specifically reach the target necrotic organ in the 4 th hours (2 03±0 60 ) and remained up to the 48 th hours (2 55±0 49) or longer, at which the 8 th was higher (4 89±0 44). Meanwhile 125 I CMHC MAb showed much higher affinity to necrotic cardiac tissue than the normal ( P <0 001). The same results were should in the blood from 4 th (4 94±0 45) to 48 th (2 70±0 12). Thus 125 I CMHC MAb can be considered as an effective tool for image diagnosis of acute myocardial necrosis.
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关 键 词: | 肌球蛋白 心肌坏死 单克隆抗体 标记 大鼠 |
ACUTE MYOCARDIAL NECROSIS BY AN IODINATED MONOCLONAL ANTIBODY OF CARDIAC MYOSIN HEAVY CHINS IN RAT |
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Abstract: | |
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Keywords: | Myosin Myocardial necrosis Monoclonal antibody |
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