Chemotactic responses of osteoblastic MC3T3-E1 cells toward zinc chloride |
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Authors: | Sanae Kanno C D Anuradha Seishiro Hirano |
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Institution: | (1) Research Center for Environmental Risk, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506 Ibaraki, Japan |
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Abstract: | Although zinc (Zn) is known to participate in bone formation, its exact role in the remodeling of this tissue has not been
fully clarified. The present study was designed to investigate whether Zn has a role at the resorptive sites in vitro. We
investigated the migration of osteoblastic MC3T3-E1 cells in response to Zn using a Boyden chamber assay. Exposure of MC3T3-E1
cells to Zn stimulated the migration of MC3T3-E1 cells. Checkerboard analysis revealed that the migration of MC3T3-E1 cells
toward Zn was a directional (chemotaxis) rather than a random (chemokinesis) motion.
Pretreatment of MC3T3-E1 cells with pertussis toxin completely blocked the chemotactic response of cells to Zn, indicating
that it is mediated by G-protein-coupled receptors. Because the bone is one of the major Zn storage sites, we suggest that
Zn released from bone-resorptive sites plays an important role in the recruitment of osteoblasts and bone renewal. |
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Keywords: | Zinc MC3T3-E1 chemotaxis bone renewal G-protein calcium platelet-derived growth factor |
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