谷氨酸生产菌T6—13的噬菌体的血清学和DNA内切酶分析

STUDIES ON THE SEROLOGY OF PHAGES OF GLUTAMATE-PRODUCING BACTERIUM T6-13 STRAIN AND THEIR DNAs ELECTROPHORESIS PATTERN AFTER DIGESTION WITH RESTRICTION ENZYMES

Glutamate-producing bacterium T6-13 strain was extensively used in our country to produce glutamic acid presently.Its phages were widespread in the environment in these factories. Phage contamination is extremely harmful in the production of glutamic acid. Sixty three strains of ph-age were isolated from 73 soil and sewage samples collected from 39 gluta-mateproducing factories. 20 examined of them belongs to Akermann Bl type under electron microscope.Four types of phage plaque were formed on the glutamate-producing bacterium. By using anti-phage sera prepared from 7 phages, the 63 phages were divided into 12 serotypes by neutralization test. The original T6-13 and its 3 mutant strains of baderia were examined for susceptibility and resistance to the 12 serotypes of phages. The mutant strain 4-210 was found to be resistant to all 12 serotypes. When the phage DNAs were digested with endonuclease BamHI, 16 kinds of electroph-oresis pattern were observed.