| 人乳头瘤病毒16型E6—E7基因体外转化NIH3T3细胞 |
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| 引用本文： | 金喘钱,孙亚洲.人乳头瘤病毒16型E6—E7基因体外转化NIH3T3细胞[J].病毒学报,1991,7(2):97-101. |
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| 作者姓名： | 金喘钱 孙亚洲 |
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| 作者单位： | 中国医学科学院肿瘤研究所 北京
(金顺钱，孙亚洲，张伟，梁克，明利华，王晓宏，王希霞，梁肖，侯芷瑛)，中国医学科学院肿瘤研究所 北京(商铭) |
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| 摘 要： |
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| 关 键 词： | 人乳头瘤病毒 NIH3T3细胞 转化 |
TRANSFORMATION OF NIH3T3 CELLS IN VITRO WITH HUMAN PAPILLOMA VIRUS TYPE16 E6-E7 GENES |
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| Jin Shunqian Sun Yazhou Zhang Wei Liang KeMing Lihua Wang Xiaohong Wang XixiaLiang Xiao Hou Zhiying Shang Ming.TRANSFORMATION OF NIH3T3 CELLS IN VITRO WITH HUMAN PAPILLOMA VIRUS TYPE16 E6-E7 GENES[J].Chinese Journal of Virology,1991,7(2):97-101. |
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| Authors: | Jin Shunqian Sun Yazhou Zhang Wei Liang KeMing Lihua Wang Xiaohong Wang XixiaLiang Xiao Hou Zhiying Shang Ming |
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| Abstract: | A fragment containing HPV16 E6-E7 ORFs with its autologous Long Control Region (HPV16 LCR E6-E7) was inserted into eukaryotic expression vector pSV2neo in sense orientation. We successfully transformed NIH3T3 cells in vitro with the constructed plasmid. The transformed cells showed anchorage-independent growth and can cause tumor in nude mice. Southern blot analysis of DNAs from the transformed cells and tumor tissues in nude mice showed integration of the constructed plasmid into the host DNA. The results indicated that HPY16 E6-E7 transforming genes might play a critical role in the development of human cervical carcinoma. |
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| Keywords: | Human papilloma virus 16 NIH3T3 cells Trans-formation Tumor in nude mice |
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