GABA Autoreceptors Are Not Coupled to Benzodiazepine Receptors in Rat Cerebral Cortex |
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Authors: | Michael J. W. Brennan |
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Affiliation: | MRC Brain Metabolism Research Group, University of the Witwatersrand and The South African Institute for Medical Research, Johannesburg, South Africa |
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Abstract: | Abstract: Depolarization-induced release of [3H] γ -aminobutyric acid ([3H]-GABA) from preloaded slices of rat cerebral cortex was inhibited by muscimol and THIP in a dose-dependent fashion. This inhibition of release was prevented by the GABA antagonists bicuculline and picrotoxin. These results confirm previous reports postulating the existence of GABA autoreceptors on GABAergic terminals. Since benzodiazapines are known to facilitate postsynaptic GABA actions, the effect of flunitrazepam on the inhibition of GABA release mediated through the autoreceptors has been examined. At a concentration of 1 μ m or 10 μ m , flunitrazepam had no effect on the IC50 values for muscimol or THIP in inhibiting stimulated GABA release. It thus seems that GABA autoreceptors are not functionally coupled to benzodiazepine receptors in rat cerebral cortex. |
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Keywords: | GABA Autoreceptors Benzodiazepine receptors Coupling |
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